Figure 5

Figure 5

Embryo and organ-specific analysis of DNA replication and mitosis in developing mouse embryos

(A) FFPE-processed embryonic day E14.5 mouse embryo and confocal tile imaging of EdU signal in DNA replicating cells multiplexed with Ki67, αSMA, phospho-histone H3^S10, and DNA labeling. αSMA immunolabelling depicts the developing musculature, heart cardiomyocytes, as well as smooth muscle cells in multiple organs and tissues (n = 3).

(B) Insets (B) show the magnification of the boxed areas using z stack confocal microscopy (a-i).

(C) FFPE-processed E14.5 developing heart and confocal tile imaging of EdU signal in DNA replicating cells multiplexed with Ki67, αSMA, and DNA labeling (n = 2). αSMA immunolabelling depicts the developing musculature, heart cardiomyocytes, and smooth muscle cells in multiple cardiac regions.

(D) Insets (D) show the magnification of the boxed areas using z stack confocal microscopy (j-m).

(E) E14.5 heart section underwent two 4-plex staining rounds: first with conjugated antibodies and WGA, then with OpenEMMU and IBEX (n = 3).