Fig. 5.

Fig. 5.

Lmx1b is required for notochord cell inflation and body elongation. (A) Confocal images of the notochord in wild type and dKO larvae immunostained for Col2a1 at 3 dpf to show notochord sheath and aid measurement of notochord width. Pink line shows where notochord width was measured from. Scale bar: 50 μm. (B) Graph showing width of notochord as a percentage of body width at 3 dpf and 5 dpf. N=7 for wild type at 3 dpf and 5 at 5 dpf; 8 for dKO at 3 dpf and 7 at 5 dpf. Student's unpaired t-test performed at each age, where *** P=0.0009 and *** P=0.0004, respectively. (C) Confocal images of BODIPY stained larvae at 3 dpf. Scale bar: 50 μm. Low power electron microscopy images of notochord in (D) wild type and (E) dKO larvae at 3 dpf. Scale bar: 25 μm. Insets (Di, Eii) show false colouring for vacuolated cells (pink); unvacuolated cells (blue); nearby sheath cells (green); notochord sheath (yellow) and intracellular contents of vacuolated cells (purple).