Fig. 1.

Fig. 1.

Two lmx1b mutant fish lines were generated using CRISPR-Cas9. (A) Schematic showing exons targeted by gRNAs (black arrowheads) in gene and protein sequences for lmx1ba and lmx1bb. HD, homeobox domain. (B) Stereomicroscope images of wild type and lmx1b mutant lines at 5 dpf. Black arrowheads indicate oedema; white asterisks show loss of swim bladder inflation in lmx1bb^−/− and dKO; white arrowhead shows body truncation in dKO. Scale bars: 250 μm. Quantification of number of larvae at 5 dpf showing (C), oedema at varying severities and (D) swim bladder inflation or non-inflation. N=19 for wild type; 38 for lmx1ba^−/−; 19 for lmx1bb^−/− and 25 for dKO. (E) Graph showing body length measurements from 1–7 dpf in wild-type, lmx1ba^−/−, lmx1bb^−/− and dKO larvae. Two-way ANOVA performed comparing to wild type, where *** P=0.009 and ****P<0.0001 for dKO; † P=0.0458 and †† P=0.0064 for lmx1bb^−/−. (F) Kaplan–Meier survival curve showing survival rate of lmx1b mutant lines compared to wild type N=14 for wt and lmx1ba mutants; 20 for lmx1bb mutants; 36 for dKO mutants.