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Fig. 4 tssk6-mRNA injection increased sperm quality in zebrafish. (A) Trimmed mean of M value (TMM) of tssk6 gene at 127 dph. (B) Effect of HT on the expression of tssk6 gene in the testis (n = 9). (C) Intraperitoneal (IP) injection technique and tssk6-mRNA synthesis (n = 7). (D) Expression of tssk6 gene. Effect of tssk6-mRNA injection on sperm quality (E) and sperm motility (F). **P < 0.01, compared with 28℃ group. #P < 0.05 and ##P < 0.05, compared with injection of Ringer's solution group. Data were presented as means ± SD.
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