Figure 3.

Figure 3.

(A) Adult mitfa^Cas9 MG-NT and MG-sox10 F0 fish. (B) Proportion of MG-NT (n=19) and MG-sox10 (n=16) F0 fish with disrupted stripes phenotype. (C) Adult mitfa^Cas9 MG-NT and MG-sox10 F1 fish. (D) The average width of melanocyte stripe 1D and xanthophore interstripe X0 was calculated for each fish by averaging 5 stripes/interstripe width measurements. N=5 fish per genotype. Two-sided Student’s t-test was used to assess statistical significance, ***p<0.001; error bars, SD. (E) F1 MG-NT (n=5) and MG-sox10 (n=5) adult fish were treated with epinephrine, and melanocytes were counted within a defined rectangular region of interest 3.46 mm × 2.54 mm encompassing the top and middle melanocyte stripes. Two-sided Student’s t-test was used to assess statistical significance, *p<0.05; error bars, SD. (F) Schematic of neocuproine (neo) experimental setup. Adult mitfa^Cas9 MG-NT (n=5) and MG-sox10 (n=5) F1 fish were treated with neocuproine for 24 hr to ablate melanocytes, then imaged at days 7, 15, and 70 to measure regeneration of melanocytes compared to day 0. Created with BioRender.com. (G) Quantification of melanocyte regeneration. Fish were treated with epinephrine prior to imaging to enable counting of melanocytes. Two-sided Student’s t-test was used to assess statistical significance, **p<0.01; error bars, SD. (H) Representative images are shown for MG-NT and MG-sox10 fish pre- and post-neocuproine treatment.