ZFIN Image: Perlee et al., 2025, Figure 2

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Figure Caption

Figure 2—figure supplement 1. <italic toggle='yes'>albino</italic> knockout fish.

(A) Quantification of pigmentation for 1 month post-fertilization (mpf) mitfa^Cas9 MG-NT and MG-albino F2 fish. Pigmented area/mm² calculated for n=12 fish/genotype within a defined rectangular region of interest (ROI) encompassing the top and middle melanocyte stripes. Two-sided Student’s t-test was used to assess statistical significance, ****p<0.0001; error bars, SD. (B) Color and GFP images of 1 mpf mitfa^Cas9 MG-NT and MG-albino F2 fish. (C) Pigmented area/mm² calculated for n=12 fish/genotype within a defined rectangular ROI encompassing the top and middle melanocyte stripes. Two-sided Student’s t-test was used to assess statistical significance, ****p<0.0001; error bars, SD. (D) Color images of 3 mpf mitfa^Cas9 MG-NT and MG-albino F2 fish. (E) Indel chart for the albino locus produced using CRISPRVariants. **T insertion is observed across all samples, likely resulting from a PCR or sequencing artifact.

Acknowledgments

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