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Fig. 4 Macrophages fight hyphae in control and posaconazole-treated larvae. Neutrophil-defective mpx:rac2D57N larvae with labeled macrophages (mpeg1:EGFP or mpeg1:H2B-EGFP) were infected with mCherry-expressing TBK5.1 A. fumigatus spores at 2 dpf and treated with vehicle control or 2.5 µg/ml posaconazole. Larvae were live-imaged every day for 5 dpi and macrophage number and fungal area were quantified. Data represent five pooled replicates. A. Example images showing macrophages surrounding hyphae. B. The number of macrophages at the infection site at 1 and 2 dpi is plotted. Each dot represents one larva, color-coded by replicate. Lines represent emmeans and error bars represent SEM. P values were calculated by ANOVA. C. For each larva that experienced germination and fungal growth, the maximum fungal area observed is plotted versus the number of macrophages at the infection site on the same day. Each dot represents one larva.