Figure 7

Figure 7

HSPC development module in HemAtlas

A. Visualization of the HSPC cross-stage atlas for each species. Cells are color-coded by their corresponding cell stages (left) and data sources (right), respectively. The pie plots showing the cell fraction of HSPCs in different developmental stages and data sources for each species. B. Dot plot showing GO terms enriched in HSPC-stage differentially expressed genes in each mouse developmental stage. The color of dot represents the adjusted P value. C. The HSPC metabolic score of representative carbohydrate metabolism and lipid metabolism pathways in three mouse developmental stages. P values were determined by Kruskal–Wallis test (****, P < 0.0001). D. Dot plot showing the stage differentially expressed immune-related genes in mouse HSPCs. The size of the dot corresponds to the percentage of cells expressing the gene in each stage. The color represents the average expression level. The immune-related genes were obtained from a recent study (pmid35418685) [11]. E. Mouse 48 stage-common and HSPC-specific TFs and their enriched GO terms. The HSPC-specific and stage-common core TFs were the intersection of HSPC specifically expressed TFs across three hematopoietic stages. The dashed red line corresponds to adjusted P value = 0.05. F. Heatmap showing stage differentially expressed TFs of mouse HSPCs in three developmental stages. The representative TFs are labeled on left side. The color represents the scaled averaged gene expression level. G. Dot plot showing the Cd44 based ligand–receptor pairs between HSPCs and their dominant regulators (the cell types with the top 2 highest numbers of interactions with HSPCs) across stages. The color of the dot corresponds to the probability of cell–cell communication; the size of the dot represents the P value of cell–cell communication. pmid, PubMed identifier number.