Figure 1

Figure 1

Scheme of HemAtlas

A. Data statistics of the multi-omics data (including bulk RNA-seq, scRNA-seq, ATAC-seq, scATAC-seq, ChIP-seq, and spatial transcriptomics data) collected in HemAtlas. B. Species/models and corresponding stages/systems of collected datasets included in HemAtlas. C. Metadata of multi-omics data collected from multiple organs/systems in multiple species/models in HemAtlas. D. Visualization of multi-omics data using a four-module platform in HemAtlas. E. Organ-wide hematopoietic references constructed for each species. F. Three single-cell featured functions deployed in HemAtlas: cross-stage analyses (i.e., online data analyses to reveal the dynamic differences in various stages during hematopoietic development for each species, encompassing distinct cell types, dynamic gene expression, and HSPC states, as well as diverse niche cells and their intercellular communications across different stages), cross-species analyses (i.e., data analyses to unveil the evolutionary conservation and differences in hematopoiesis), and cross-model analyses (i.e., online comparisons between user-uploaded in vitro HSPC scRNA-seq data and available in vivo omics data to provide insights for engineering the hematopoietic system in vitro). G. The featured functions in (F) aim to reveal more biological insights into hematopoiesis both in vitro and in vivo. H. The HSPC development module constructed in HemAtlas, which aims to decode HSPC cross-stage development across species. AGM, aorta–gonad–mesonephros; FL, fetal liver; BM, bone marrow; PL, placenta; CHT, caudal hematopoietic tissue; KM, kidney marrow; RNA-seq, RNA sequencing, scRNA-seq, single-cell RNA-seq; ATAC-seq, assay for transposase-accessible chromatin with sequencing; scATAC-seq, single-cell ATAC-seq; ChIP-seq, chromatin immunoprecipitation followed by sequencing; HSPC, hematopoietic stem and progenitor cell.