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Fig. 8 Liver, Brain histology and Biochemical analysis of gut tissue of mice. (A–D) Biochemical analysis of total protein, MDA content, GSH content and catalase activity of gut tissue of mice, respectively. Mean values in each column do not differ significantly at p < 0.05. Comparison between control and treatment mean values was made by Dunnett’s multiple comparisons test. Where, “****” = p < 0.0001, “ns” = data is not significant. Data were subjected to analysis of variance (ANOVA one way) test by using the GraphPad Prism 8.0.1 version software. (E) Control liver having normal hepatic lobules. (F) is the liver section of HFD fed mice having lipid deposition (black circle) in intracellular space of hepatic cells. (G) is the liver section of probiotic fed mice with almost normal histoarchitecture having reduced number of lipid droplets (arrow heads) than the former. (H) Control brain depicting clear granular layer (G), molecular layer (M) and white matter (WM); (I) brain tissue section of HFD fed mice is having vacuolated (V) white matter while in case of HFD + RSE02 fed mice brain. (J) the reduced size and numbers of vacuoles in white matter were observed. (K-M) are images of white matter of control, HFD fed and HFD + RSE02 fed mice, respectively where L is having vacuolated white matter but the condition became better in M.