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Fig. 5 (AI–IV) Cell adhesion assay of RFP-labelled RSE02 in Caco-2 cell line where, AI: Phase contrast, AII: DAPI staining, AIII: merge image of AI & AII and AIV: TRITC filter respectively under 40X magnification. (B) Kaplan-meir survival curve of zebrafish on exposure to RSE02 strain (PBS: Control, dose 1: 1 × 109 CFU/mL, dose 2: 1 × 1010 CFU/mL and dose 3: 2 × 1010 CFU/mL). (C) Cell cytotoxicity of Caco-2 cell line on exposure RSE02 bacterial cell free supernatant (control: PBS, 10, 20 and 30 μL csf: 1 × 109 CFU/mL bacteria. (DI & II) Transverse section of gut through the intestinal bulb segment showing RFP-labelled RSE02 (red) and nuclear DAPI staining (blue) in Control (PBS exposure) and RSE02-exposed adult zebrafish after 7 day treatment; GL, Gut lumen; Magnification 10x; Scale bar 100 μm. (EI–IV) Microscopic visualization RSE02 in zebrafish gut smear where, EI & II: gut smear of untreated fish under both TRITC & FITC filter and EIII & IV: gut smear of treated fish under both TRITC & FITC filter.