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Fig. 4 - Supplemental 2 Notch reporter (Tp1) expression. (A) Images before and after treatment with DMSO and LY411575 (Notch inhibitor) in the Tg(Tp1:EGFP). (B) Treatment with LY411575 resulted in a significant overall reduction in Notch expression levels in the TP1 Notch reporter line compared to the DMSO control group (p<0.001, N=5 larvae per group). (C) Images before and after cytoneme inhibition through drug induction of cdc42DN-expression in krt4+ keratinocytes and direct inhibition of Notch signaling via drug induction of SuHDN expression in krtt1c19e+ keratinocytes, along with DMSO treatment. (D) The number of Tp1+ keratinocytes peaked during epidermal remodeling (6.5–7.5 SSL, N=58 larvae total) in zebrafish. (E) A significantly lower number of Tp1+ keratinocytes was observed in Tg(krt4:il17rd) or Tg(krt4:il17r1a1) compared to control (Figure 5C). Statistical significance was assessed by Student t test. Scale bars: 100 µm (A, C, E). Error bars indicate mean ± SEM.