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Fig. 1 Differentiated keratinocytes extend cytoneme-like cellular protrusions. (A) Airineme-like protrusion (arrowhead), and (B) cytoneme-like protrusions (arrowheads) labeled in krt4:palmEGFP injected keratinocytes. (C) Keratinocytes expressing krt4 extend cytoneme-like protrusions most frequently during metamorphic stages (6.5–7.5 SSL; N=457 cells, 19 larvae total). A cytoneme-like protrusion (arrowhead) labeled for (D) F-actin, revealed by LifeAct-mRuby but did not colocalize (E) with tubulin, revealed by Tuba-mCherry. (F) Keratinocyte cytonemes extend faster than they retract (N=125 cells, 4 larvae total), with an average length (G) of 18.21 µm (N=190 cells, 4 larvae total). Scale bars: 20 µm (A, B, D, E). Error bars indicate mean ± SEM.