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Fig. 6 - Supplemental 3 clint1 regulates epidermal maintenance via keratinocyte cytonemes. (A–A’) clint1 mutants exhibit a disorganized periderm. (B–E) clint1 mutants show (B) a lower frequency of cytoneme extension as compared to controls (WT, cx41.8-/-) (F2, 186 = 14.55, p<0.0001, N=10 larvae total), (C) reduced Notch responsiveness (p=0.0119, N=12 mutants, N=10 controls), (D) increased krtt1c19e expression in the periderm (p<0.0001, N=13 mutants, N=6 controls), and (E) a greater number of keratinocytes within the periderm (p<0.001, N=8 mutants, N=6 controls). Statistical significances were assessed by One-way ANOVA followed by Tukey’s HSD post hoc test and Student t test. Scale bars, 20 µm (A–A’). Error bars indicate mean ± SEM.