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Figure Caption
Fig. 6 - Supplemental 1 CRISPR/Cas9 induced knockout of zebrafish il17a resulting in a premature stop codon. (A) Comparison of Sanger sequencing chromatograms between wild-type and heterozygous il17a mutant zebrafish. The region targeted by the single guide RNA is highlighted within the black box. (B) Sanger sequencing of the il17a mutant reveals an 11 bp deletion, leading to the induction of a premature stop codon. (C) Western blotting confirmed that IL-17 protein expression was not detected in the homozygous il17a CRISPR mutants.
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