Figure 5

Figure 5

Eupatilin but not Rock inhibitor increases ciliation in NPHP5 URECs. (a–d) Control (1.56NC) and NPHP5 (2.05P1) URECs treated with DMSO (0,04%) or either ROCK inhibitor (Y-27632i, 25 μM; a and b) or Eupatilin (EUP, 20 μM and/or 40 μM; c and d) were fixed and stained primary cilia (ARL13B, green) and basal body markers (γ- tubulin, red; a and c). Ciliogenesis was quantified similarly as in Figure 1 (EUP, b; Y-27632, d). (b) n = 3 to 5 experiments, ∗∗ P < 0.05. (d) n =2 to 4 experiments. The statistical analysis were performed using R script for mixed linear-regression model with quasibinomial penalization. DMSO, dimethyl sulfoxide; EUP, Eupatilin; UREC, urinary renal epithelial cells.