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Fig. 2 Spectral response properties of NAHCP for H2S. (a) UV-visible absorption spectra of 10 μM NAHCP, 10 μM dye 3, and 10 μM NAHCP + 100 μM H2S; (b) the fluorescence response spectrum of the 10 μM NAHCP toward different concentrations of H2S (0–100) μM; (c) the fluorescence intensity of I533 change vs. different concentrations of H2S (0–100) μM; (d) the I533 of the 10 μM NAHCP responded linearly to the range from 0 to 10 μM H2S; (e and f) the bright-field imaging of 10 μM NAHCP in the absence and the presence of 100 μM H2S, respectively, the embedding graph in picture (a); (g and h) the 365 nm UV-lamp radiated fluorescent imaging of 10 μM NAHCP in the absence and the presence of 100 μM H2S, respectively, the embedding graph in picture (b). All detection systems in 10 mM PBS buffer (5% DMSO, v/v, pH 7.4) at 37 °C, n = 3.