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Fig. 3 Inhibition of the muscle clock disrupts circadian variations in proteasomal flux in the muscle. (A) Effect of muscle clock inhibition on circadian accumulation of MuRF in control and mΔCLK sibling larvae under free-run between 3 and 4 dpf. MuRF and Myc-tagged ΔCLK abundance were normalized to total protein. Statistics are two-way ANOVA (Time/Genotype) with Bonferroni’s post hoc test. (B) Effect of muscle clock inhibition on circadian variations in proteasomal degradation of ubiquitinated proteins in control and mΔCLK sibling larvae under free-run between 3 and 4 dpf treated from CT0 with either DMSO or bortezomib (BTZ), and protein analyzed by western blotting at CT3 and CT15. Statistics are three-way ANOVA (Time/Genotype/Treatment) with Bonferroni’s post hoc test. (C and D) Circadian myotome growth between 3 and 4 dpf (C) or tail displacement at 4 dpf (D) measured in control and mΔCLK sibling larvae under free-run that were treated from CT0-24 with either DMSO, Myomed, or bafilomycin A1 (Baf A1). Images (D) are superimposition of video frames. Statistics are two-way ANOVA (Time/Treatment) with Bonferroni’s post hoc test.