Fig. 2

Fig. 2 Affinity of the different nanosystems towards different mycobacteria.

a Representative flow cytometry analysis of different strains of mycobacteria incubated with PBS (gray), or 200 µg/mL of MSN (blue), MSN-TPP (yellow), MSN-AVA-TPP (orange) and MSN-AVA₂-TPP (pink). Histograms were generated on the bacterial population expressing mScarlet, tdTomato or mCherry, detailed in the gating strategy (Supplementary Fig. 1). b Percentages of FITC-positive mycobacteria incubated with 200 µg/mL of each functionalized nanosystem. Data represent 3 biological replicates with 2 technical replicates each with at least 1 million events, and are shown as mean ± SD (n = 6). Comparisons between functionalized nanosystems and control MSN were done using ordinary one-way ANOVA with Dunnett’s multiple comparisons test. Adjusted p values (from left to right): M. marinum, M. fortuitum, M. smegmatis: ****p < 0.0001; M. avium: **p = 0.0011 (TPP), ***p = 0.0002 (AVA), ***p = 0.0006 (AVA₂); M. kansasii: *p = 0.0142 (TPP), **p = 0.0095 (AVA), *p = 0.0320 (AVA₂). c Representative Scanning Electron Microscopy (SEM) micrograph of Mmar cells after incubation with each nanosystem. The white arrows point out individual MSN. Scale bar: 2 µm. Two independent experiments were performed. Source data are provided as a SourceData file.