|
Fig. 3 Gastrointestinal stability of EPF3 in SGF, SIF, and HC2C. EPF3 was incubated with 3 buffers respectively. Samples in SGF (A and B), SIF (C), and HC2C (D) were taken at 0, 60, 120, 240, and 360 min and were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The relative content of EPF3 in SIF (E) and HC2C (F) at each time point was calculated. Data were presented as mean ± standard deviation (n = 3). nsP > 0.05, vs the Con group. Con: control group; EPF3: a fibrinolysin monomer isolated and purified from Pheretima vulgaris Chen; HC2C: homogenate of Caco-2 cells; M: protein molecular weight standard marker; SGF: simulated gastric fluid; SIF: simulated intestinal fluid.