|
Fig. 6 Dosage-dependent regulation of microglia maintenance by Pu.1/Spi1 is evolutionary conserved in mice. (A) The experimental setup for Spi1 conditional knockout in adult mice. (B) Representative images of IBA1 and DAPI co-staining in the cortex, hippocampus, and thalamus of Spi1Fl/+;Cx3cr1CreER and Spi1Fl/Fl;Cx3cr1CreER mice at 7 dpi. (C) Quantification of the density of IBA1+ microglia in the cortex, hippocampus, and thalamus of Spi1Fl/+;Cx3cr1CreER (n=3) and Spi1Fl/Fl;Cx3cr1CreER (n=3) mice at 7 dpi. (D) The experimental setup for conditional knockout of Spi1 in Spi1Fl/+;Cx3cr1CreER mice, and the subsequent PCR detection and T-clone quantification of Spi1Fl and Spi1KO alleles in sorted YFP+ microglia. (E) Gel image shows the relative intensity of amplified DNA bands of Spi1Fl and Spi1KO alleles in microglia sorted from Spi1Fl/+;Cx3cr1CreER mice at different stages post-tamoxifen (TAM) injection. (F) Quantification of the percentage of Spi1KO allele at 3 dpi (n=4) and 3.5 mpi (n=4) by T-clone assay. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.