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Fig. 2 - Supplemental 1 Characterization of pu.1KI and pu.1CKO alleles. (A) Summary of the efficiency for the generation and screening of pu.1KI. (B) Gel image shows the amplified 5‘- and 3’-junction of the donor plasmid integration site in pu.1KI allele. (C) Summary of the genomic DNA sequences of the 5’- and 3’-junction in pu.1KI allele from different F0 founders. (D) Quantitative RT-PCR shows the relative expression of DsRed and pu.1 from pu.1CKO allele.
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