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Fig. 2 - Supplemental 1 Phenotypes of epha4a mutants. (A) Representative images showing the notochord and external phenotypes of wild-type and epha4a mutants. The red dashed lines indicate the area of the notochord. (B) Whole-mount in situ hybridization results showing the expression of the rfng gene in 18 hr post-fertilization (hpf) wild-type, homozygous mutant, or morphant larvae as indicated. The rfng gene marks boundary cells in the hindbrain. The red arrows indicate boundary expression. The numbers of embryos analyzed are shown on the top right. Scale bars: 200 µm in panel (A), 100 µm in panel (B).