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Fig. 6 Decreased aggressiveness, reduced social cohesion, and impaired social interaction in pccbe3/+ zebrafish.A Schematic of the aggressiveness test and representative heatmaps (n = 10), showing zebrafish behavior in response to their mirror image. B Ratio of distance traveled in the biting zone (adjacent to the mirror) to the total distance moved, indicating reduced aggressiveness in pccbe3/+ zebrafish. C Ratio of time spent in the biting zone to the total time, supporting findings of decreased aggressiveness. D Schematic of the shoaling test with representative tracking plots. Each color represents an individual fish in the control group (pccb+/+, n = 28; pccbe3/+, n = 20), illustrating social cohesion. E Time spent near other fish without direct contact, reflecting social interest without physical engagement. F Time spent in direct body contact with other fish, indicating levels of social interaction. G Average inter-individual distance between fish, measuring social spacing and cohesion. H Schematic of the social preference test and representative heatmaps (n = 20), assessing the tendency of zebrafish to engage with conspecifics. I Ratio of distance traveled in the social zone (area adjacent to conspecifics) to the total distance moved, indicating social preference. J Ratio of time spent in the social zone to the total time, demonstrating impaired social interaction in pccbe3/+ zebrafish compared to controls. Bars represent the mean ± SEM.