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Fig. 3 gal Exerts a modest effect on seizure activity in eaat2a-/- mutants. (A) Averaged calcium signals (elavl3:GCaMP5G) for spontaneous seizures recorded across the brain of 5 dpf control (eaat2a-/-) larva (blue, 35 events) and eaat2a-/-;hsp70l:gal (orange, 39 events) aligned by 50% of maximum amplitude. Shaded area represents SEM. (B) Time to peak calculated from beginning of aligned seizure until maximum ΔF/F0 signal. (C) Area under the curve (AUC) calculated over spontaneous seizures normalized to control (n control = 35; n eaat2a-/-;hsp70l:gal = 39). (D) Number of spontaneous seizures per larva. (E) Amplitude of spontaneous seizures per larva. (F) Duration of spontaneous seizures per larva. (G) Averaged calcium signals (elavl3:GCaMP5G) for spontaneous seizures recorded across the brain of 5 dpf control (eaat2a-/-) larva (blue, 77 events) and eaat2a-/-;gal-/- (yellow, 89 events) aligned by 50% of maximum amplitude. Shaded area represents SEM. (H) Time to peak calculated from beginning of aligned seizure until maximum ΔF/F0 signal. (I) AUC calculated over spontaneous seizures normalized to control (n control = 77; n eaat2a-/-;gal-/- = 89). (J) Number of spontaneous seizures per larva. (K) Amplitude of spontaneous seizures per larva. (L) Duration of spontaneous seizures per larva. Significance levels: ***p<0.001, **p<0.01, *p< 005, ns = not significant (p>0.05), Wilcoxon-Mann-Whitney test (B, C, E, F, H, I, K, L), negative binomial regression (D, J).