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Fig. 2 - Supplemental 2 Immunostaining confirms protein deficiency in gal -/- mutants whole-mount immunostaining of galanin localized in the diencephalon of 5 dpf zebrafish larvae. Antibodies used for staining included anti-galanin (green) and anti-ac. tubulin (acetylated tubulin, magenta). (A1) representative galanin staining in control larvae. (A2, A3) Single immunohistochemical channels. (B1) representative galanin staining in gal -/- larvae. (B2, B3) Single immunohistochemical channels. Scale bar = 25 μm
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