Fig. 5 Single-cell RNA-sequence analysis reveals different gene features in grem1a-knockdown cardiomyocytes of zebrafish at 48 hpf. A UMAP plot of myocytes from control and grem1a-knockdown embryos. B The levels of cardiomyocyte-related marker gene in cell clusters from (A). C Differential gene expressions (DGEs) of grem1a-KD cardiomyocytes versus control cardiomyocytes. D GO analysis of genes in control and grem1a-knockdown cardiomyocyte. E UMAP plot of cardiomyocytes from control and grem1a-knockdown embryos. F The percentage of cells from different group in three cardiomyocyte clusters. G KEGG analysis of top 100 DEGs among three cardiomyocyte clusters. H Bubble plot of enriched genes in three cardiomyocyte clusters. I Genes that have significant change in grem1a-KD cardiomyocytes versus control cardiomyocytes. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 vs control
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