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Fig. 2 TCE exposure down-regulated the level of grem1a in zebrafish. A Volcano plot and B hot map of DEGs analysis of bulk RNA sequencing from 12 hpf zebrafish embryos. C GO enrichment terms (above) and KEGG pathways (below) enriched from DEGs. D Relative mRNA expression change of 12 hpf zebrafish embryos measured by RT-qPCR. E Whole-mount in situ hybridization of gene grem1a in 72 hpf zebrafish (n = 15). The hearts were circled by white dashed line and indicated by red arrows. Ctrl, control. TCE, trichloroethylene, 760 nM. ***, P < 0.001 vs control
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