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Fig. 5 CCCP treatment reduces ROS in adult CM. (a,b) Representative immunofluorescent images showing 123-DHR staining, along with a bar graph quantifying the fluorescence intensity of 123-DHR in the CCCP-treated group compared to control. (c,d) Representative immunofluorescent images for CellROX and the corresponding bar graph quantifying the fluorescence intensity of CellROX in the CCCP-treated group versus control. (e,f) Representative immunofluorescent images for VDAC, along with a bar graph quantifying VDAC fluorescence intensity in the CCCP-treated group compared to the control. Scale bar = 50 µm or as indicated. Data are presented as mean ± SE; *** = p ≤ 0.0002; **** = p ≤ 0.0001. Statistical significance was determined using an unpaired t-test, with p ≤ 0.05 considered significant. A minimum of 30 cells per experimental group were analyzed per experiment, and the experiment was repeated at least three times. White dotted lines delineate the boundaries of individual CMs.