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Fig. 4 Increased proliferation and mitotic activity in CCCP-treated adult CM: Representative immunofluorescent images showing adult CMs positive for (a) KI67, (c) PH3, and (e) AuroraB in the CCCP-treated group compared to the control. The corresponding bar graphs quantify the proportion of (b) KI67-, (d) PH3-, and (f) AuroraB-positive CMs in the CCCP-treated group compared to the control. White arrow indicates the adult cardiomyocyte positive for KI67, PH3, or AuroraB. Scale bar = 50 µm. Data are expressed as mean ± SE; **** = p ≤ 0.0001. Statistical significance was determined using an unpaired t-test, with p ≤ 0.05 considered significant. A minimum of three images per group per trial were analyzed, and the experiment was repeated at least three times.