ZFIN Image: Feng et al., 2025, Fig. 1.

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Figure Caption

Fig. 1.

Dynamic expression of Nr4a1 after cardiac injury. (A) Expression pattern of nr4a1 at different stages post-injury. The fold change is calculated relative to the expression in uninjured wild-type (wt) hearts. (B) The schematic for generating TgKI(nr4a1:eGFP). (C) Detection of nr4a1:eGFP fusion mRNA. The 518 bp band indicates correct nr4a1:eGFP fusion cDNA. (D) Western blot analysis of TgKI(nr4a1:eGFP) to identify the fusion Nr4a1:eGPF protein (88kD). (E-H) Expression of Nr4a1, as revealed by GFP antibody staining, in 2 and 7 dpci hearts. (I) IB4 staining to localize Nr4a1 expression in macrophages within 7 dpci hearts. (J) Anti-Mpx antibody staining to localize Nr4a1 expression in neutrophils within 2 dpci hearts. P-values<0.05 were considered statistically significant (two-tailed unpaired t-test). Scale bars: 60 µm.

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