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Fig. 1 Rationale for PerCell-normalized semi-automated ChIP-seq analysis Shown are conceptual representations and comparisons of ChIP results after analyzing cells of varying ploidy (1n, 2n, and 4n) via conventional spike-in or PerCell normalization. The PerCell methodology specifically enables the detection of changes in relative signal across cellular backgrounds with different chromatin content and the use of a single antibody.
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