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Fig. 7 - Supplemental 2 Haploinsufficiency of bcas2 does not affect pre-mRNA splicing during primitive hematopoiesis. (A) The number of five major types of alternative splicing events was analyzed from RNA sequencing data. Embryos were lysed at the 10-somite stage and subjected to reverse transcription. The cDNA library was prepared, sequenced and then analyzed using rMATS. The five main alternative splicing types refer to exon skipping (SE), alternative 3’ splicing site (A3SS), alternative 5’ splicing site (A5SS), intron retention (RI), and mutually exclusive exons (MEX). (B) Examples of different types of alternative splicing were analyzed by reverse transcription PCR using total RNA from sibling and bcas2+/Δ14 embryos at the 10-somite stage. (C) Reverse transcription PCR analysis of total mdm4-FL and mdm4-S isoforms in bcas2+/Δ14 and sibling embryos at the 10-somite stage. (D) Analysis the pre-mRNA and mature mRNA of β-catenin in bcas2+/Δ14 and sibling embryos at the 10-somite stage.