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Fig. 5 - Supplemental 1 BCAS2 inhibits the nuclear export of β-catenin. (A–B’) GFP-tagged S37A-β-catenin was co-expressed with or without BCAS2 in HeLa cells. The entire nucleus (A) or entire cytoplasm (B) was bleached. (A, A’) Representative images at the indicated timepoints (A) and the related fluorescence recovery curves (A’, n=7) showing the kinetics of nuclear import of S37A-β-catenin. (B, B’) Time-lapse imaging (B) and recovery curves (B’, n=7) from cytoplasmic photobleaching experiments reflecting the kinetics of nuclear export of S37A-β-catenin. (C) Quantitative analysis of fluorescence recoveries after photobleaching (n=7). ns, not significant, **p<0.01 (Student’s t-test). Scale bars, 10 μm (A, B).