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Fig. 3 - Supplemental 4 Knockdown of bcas2 significantly reduces nuclear β-catenin in the primitive myeloid cells. (A) Immunofluorescence staining of β-catenin in Tg(coro1a:GFP) embryos at 17 hpf. The embryos were injected with 8 ng of the indicated MOs at the one-cell stage and then collected for immunofluorescence staining. The dotted lines refer to the GFP-positive primitive myeloid cells. The relative fluorescence intensity of β-catenin was quantified in (B) (n=6). **p<0.01 (Student’s t-test). Scale bars, 10 μm (A).
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