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Fig. 4 Pictorial and Graphical representation of anti-angiogenic effect of CIS, ZER, and Combination. A The larvae were exposed to CIS, ZER, and their Combination dose stained at 72 hpf with O-dianisidine staining to detect RBCs in specific areas such as dorsal aorta (DA), inter-segmental vessels (ISV), dorsal longitudinal anastomotic vessel (DLSV), and posterior cardinal vein (PCV). Note the greatest decrease in RBC stained cells in combination treated larvae than individually ZER and CIS treated larvae. B Graphical representation indicates that in ZER and Combination treated larvae O–Dianisidine score is extremely significant (p < 0.001) than CIS treated larvae