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Fig. 1 Effect of CIS, ZER, and Combination treatment on mortality at 96hpf and combination index plot to predict effect of drug in Zebra fish model. A–C indicates the acute toxicity of CIS, ZER, and Combinational dose in zebra fish larvae, dose dependent increase in mortality was observed irrespective of the treated groups compared to control, LC50 value of CIS and ZER was found to be 43.970 μg/ml CIS, 20.83 μg/ml ZER. In combination treated larvae only 25%, 10% mortality was observed at highest concentration. D Combination index value was calculated through Compu-Syn software (1.0), in accordance to the Chou and Talalay (1984) method. CI reaction was obtained by plotting the fraction affected (fa) against the CI plot to predict synergistic, agonistic and antagonistic effects of the drug in zebra fish model