Fig. 6

Fig. 6 pax6a/vegfaa co-expressing cells guide central arteries migration.

The angle of the developing CtA 1 (a), CtA 2 (b), CtA 3 (c), CtA 4 (d), CtA 5 (e), CtA 6 (f), and CtA 7 (g) were measured using the PHBC as the basis (0 degrees). The angle was measured before the turnover of the CtA towards the BA or directional change of the CtAs towards its ipsilateral neighbors. CtAs and pax6a/vegfaa co-expressing cells that were absent are indicated as 0°. Grey boxes show the CtAs that changed the directional migration during hindbrain vasculature development. a–g Statistical significance was determined using 2-way ANOVA. n = 8 (30 hpf CTRMO, 32 hpf MCT8MO), 9 (30 hpf MCT8MO, 36 hpf MCT8MO), 10 (all other stages and conditions). Data are presented as box-and-whisker plot, where the black thick horizontal line represents the median. Each dot represents a biological replicate; error bars represent standard deviations (smallest and highest value). For detailed statistics, see Supplementary Data 2. h Graphical representation of the CtA directionality and the position of the pax6a/vegfaa co-expressing cells during BHB development. The mean values were used to construct these graphs.