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Figure Caption
Fig. 1 Comparison of EE and IF obtained for each target by microinjection of Alt-R HDR components with 100, 200, 400 or 800 pg Cas9 into the yolk of one-cell-stage zebrafish embryos. Left-hand y axes show EE (blue) and right-hand y axes show IF (gray). The bars and error bars represent the mean + s.e.m. of three biologically independent replicates (n = 3), where each replicate represents a pool of 20–30 injected embryos. Tukey’s multiple-comparisons test: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
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