Figure 1

Figure 1

CCCP treatment decreases mitochondrial mass in adult CM. (a) Schematic overview of the in vitro experimental design, showing the isolation of adult CMs, parkin transfection, CCCP treatment, and subsequent proliferation and oxidative stress analysis. (b) Representative images of MitoTracker staining and (c) TMRM staining, demonstrating a decrease in mitochondrial mass in CCCP-treated adult CMs compared to control. Bar graphs (d,e) quantitatively show MitoTracker and TMRM staining intensities in CCCP-treated CMs versus control. Scale bar = 50 µm. Data are presented as mean ± SE; *** = p = 0.0003; **** = p < 0.0001. Statistical significance was determined using an unpaired t-test, with p ≤ 0.05 considered significant. A minimum of 30 cells per experimental group were analyzed per experiment, and all experiments were repeated at least three times. White dotted lines delineate the boundaries of individual CMs.