Figure 3

Figure 3

Graphics of sublethal effects induced by different concentrations of N-Borate FR in the zebrafish embryo toxicity test. Specifically, delayed yolk sac absorption (a) and yolk sac darkening (b) were observed. Statistical analysis was conducted by comparing exposed groups to the control, with significance levels denoted as follows: ** (p ≤ 0.01), and *** (p ≤ 0.001). Data are presented as mean ± standard deviation. Data visualization was performed using SigmaPlot^® software, version 14.0. Sublethal effects observed in embryos exposed to Phosphate- FR (2.5× and 5× magnification, Stemi2000-C stereomicroscope): (c) normal embryo and embryo exhibiting delayed yolk sac absorption (c1); (d) normal larvae in comparison with larvae showing yolk sac darkening (d1) and delayed yolk sac absorption (d2).