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Fig. 1 Characterization of GCaMP-hiCMs in vitro. Cryopreserved GCaMP-hiCMs were defrosted and cultured in vitro before transplantation. (a) Immunofluorescence staining for cardiomyocyte marker protein cTnT on day 7. Nuclei are stained with DAPI fluorescence. Scale bar = 200 μm. (b, c) Beating of GCaMP-hiCMs at 14 days after seeding in culture dishes. Vertical axis indicates intensity of fluorescence emission by cells in arbitrary units. White circle shows the analyzed area. cTnT, cardiac troponin T; DAPI, 4’,6-diamidino-2-phenylindole; GCaMP-hiCMs, genetically encoded fusion of green fluorescent protein with calcium-binding protein calmodulin expressed in human induced pluripotent stem cell-derived cardiomyocytes. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)