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Fig. 2 Immunofluorescence staining of the four newly identified HSCR-associated genes showing colocalization of expression in human ENS. Representative immunofluorescence confocal images of HAND2, JAG1, ZNF25 and UNC5C (green) in the intestine tissues of non-HSCR controls, showing colocalization with TUJ1 (red) in enteric neurons in the ganglion cells of the submucosal and myenteric plexuses. DAPI nuclear staining is also shown (blue). The images at right panel represent the zoomed-in views of selected ganglion cells in submucosal (upper right) and myenteric (bottom right) plexuses as indicated by the dotted boxes. m: mucosa; sm: submucosa; cm: circular muscle layer; lm: longitudinal muscle layer. m, mucosa; sm, submucosa; cm, circular muscle; lm, longitudinal muscle.