IMAGE

FIGURE 2

ID

ZDB-IMAGE-250609-56

Genes

aqp1a.1

Antibodies

Ab1-aqp1a.1

Publication

Mesika et al., 2025 - Impaired Proteostasis is Linked to Neurological Pathology in a Zebrafish NGLY1 Deficiency Model

All Figures

Figures for Mesika et al., 2025

Image

Figure Caption

FIGURE 2

Adult ngly1^(−/−)fish feature cellular effects resulting from NGLY1 deficiency. (A) RT‐qPCR quantification of aqp1a.1 mRNA levels in zebrafish eye. ** T‐test p < 0.01 (ngly1^(+/+)n = 3, ngly1^(−/−)n = 3). (B–E) Western blot analysis of aqp1a.1 protein from adult zebrafish eyes (B, C); *** T‐test p < 0.001 (ngly1^(+/+)n = 3, ngly1^(−/−)n = 3), and brain (D, E) ** T‐test p < 0.01 (ngly1^(+/+)n = 3, ngly1^(−/−)n = 3). β‐actin was used as a loading control. (F–I) Transmission electron microscopy images showing mitochondrial fragmentation in the mutant zebrafish brain (white arrows). (J) Fragmented mitochondria were quantified by Form Factor metric, calculated by the formula: Form Factor = (4 × π × mitochondrial area)/(mitochondrial perimeter) [2]. ** T‐test p < 0.01, (N = 3 in each group).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Inherit. Metab. Dis.