Fig. 2

Fig. 2

Bcas3 KO in zebrafish results in developmental deficiency. (A), Time-matched bright field images of WT and bcas3^−/− embryos during early development. Scale bars: 200 μm. (n = 15, per group). (B-C), Microcephaly index (the interocular distances/body length) comparison of 5 dpf (days post fertilization) WT and bcas3^−/− larvae. Representative images (B) and quantitative analysis (C) of the interocular distances/body length in 5 dpf WT (n = 37) and bcas3^−/− larvae (n = 41). Scale bars: 500 μm. (D-E), Body length comparison of 5 dpf WT and bcas3^−/− larvae. Representative images (D) and quantitative analysis of body lengths (E) in 5 dpf WT (n = 37) and bcas3^−/− larvae (n = 41). Scale bars: 200 μm. (F-G), Body length comparison of 4 mpf WT and bcas3^−/− larvae. Representative images (F) and quantitative analysis of body lengths (G) in 4 mpf zebrafish. Scale bars: 200 μm. (n = 5, per group). (H), Representative images of the brain of 4 mpf zebrafish. (I), The ratio of brain/body weight of WT and bcas3^−/− zebrafish at 4 months. (n = 5, per group). Data are shown as mean ± SEM. Unpaired Student’s t-test was used to analyze interocular distances, body length, and the ratio of brain/body weight. Significance levels are denoted as follows: *P < 0.05, ***P < 0.001