Figure 2

Figure 2

RMS tumours induce neo-vascularisation from proximal vessel beds. (A), Schematic illustrating the calculation of sub-intestinal, intersegmental and tumour induced neo-vessel lengths, by Fiji image analysis. An image of a 120 hpf (70 hpi) zebrafish xenograft in flk1-GFP background is shown with blood vessels shown in green and the RMS tumour in red. From this, the green fluorescence channel is taken, GFP +ve vessels skeletonised and their lengths measured. (B), Bar graphs showing the proportion of xenografts with neo-vessel induction for xenografts generated from each RMS cell line (left) and the average neo-vessel length for xenografts generated from each RMS cell line (right). (C, D), Bar graphs showing the average sub-intestinal vessel length (excluding neo-vessels) (C) and the average intersegmental vessel length (D) for xenografts generated from each RMS cell line. Dotted line indicates level of vehicle only injected zebrafish controls. * P<0.05, *** P<0.001, Ordinary one-way ANOVA with Tukey’s multiple comparison’s test, Data are presented as mean values +/- SEM. n = number of xenografts (except for B left, which is number of experiments).