ZFIN Image: Zhang et al., 2025, Figure 5

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Figure Caption

Figure 5 Rbm24a interacts with Buc.

(A) Scatter plot of enrichment to relative intensity (see “Methods”). Blue dots designate proteins with more than a threefold enrichment. Red dots highlight previously known Rbm24a-binding proteins and protein components of the germ plasm. (B) GO analysis of Rbm24a-interacting proteins. The hypergeometric test is employed for statistical assessment, and the Benjamini–Hochberg (BH) method is utilized for P value adjustment. (C) Full-length and truncations of Rbm24a. (D) Co-IP analysis shows the interaction between Buc and the C-terminal region of Rbm24a. (E) Full-length and truncated versions of Buc. (F) Co-IP shows the interaction between Rbm24a and a disordered region (DR3) of Buc. (G) Colocalization of Rbm24a with Buc in HEK293 cells. Scale bar, 10 μm. (H) Mutation of Buc eliminates the phase-separated pattern of Rbm24a-GFP in a 1-cell stage embryo. Scale bars, 50 μm. Source data are available online for this figure.

Acknowledgments

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