Figure 3

Figure 3 Loss of maternal Rbm24a specifically causes the dispersion of germ plasm mRNAs from phase-separated structures.

(A) Distribution of germ plasm mRNAs in wild-type and Mrbm24a embryos at the 4-cell stage. Scale bar, 200 μm. (B) FISH combined with confocal imaging showing the dispersal of germ plasm mRNAs in Mrbm24a mutants. Scale bar, 20 μm. (C, D) The absence of maternal Rbm24a disrupts the localization of germ plasm mRNAs but does not affect the phase separation of germ plasm proteins. A animal pole, V vegetal pole. Scale bars, 20 μm. (E) Similar distribution of germ granules in wild-type embryos and Mrbm24a mutants. For wild-type n = 6294, For Mrbm24a mutants n = 6332. (F) Loss of maternal Rbm24a does not affect the number of germ granules (n = 3 independent biological samples). Values are means ± SD. ns, not significant, unpaired Student’s t test. (G) Rbm24a-deficient germ granules fail to form large aggregates along the cleavage furrows. Magenta and green are pseudocolors designating Piwil1 protein and nanos3 mRNA, respectively. Scale bar, 20 μm. Source data are available online for this figure.