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Fig. 4 Bacteriolytic effects of the StM23_CWT chimera in food production environment relevant conditions. (A) Lytic activity [%] of the enzyme on L. monocytogenes strains isolated from food processing environment by Nofima, compared to the DSMZ strain. (B) Bactericidal effect (LOG CFU/coupon reduction) of the chimera on L. monocytogenes MF7703 biofilm at 1 µM and 5 µM concentrations treated for 24 h at 12 °C. Nisin at 4 ppm was used as a control and for synergy studies. Data are presented as mean ± SD of three replicates. Statistical analysis was performed using one-way ANOVA with post hoc Tukey test. Significance levels are indicated as follows: *p < 0.05, **p < 0.01. (C) Results of enzymatic surface decontamination of L. monocytogenes MF7703 on glass, stainless steel, and silicone surfaces treated with 1 µM enzyme for 1 h at room temperature.