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Fig. 3 - Supplement 1 Paralysis regulates gene expression of matn1, klf2a, and mxra5b in developing embryos. Bar plots showing global changes in relative expression from RT-qPCR of matn1, klf2a, and mxra5b genes between WT and cacnb1−/− mutant embryos at 48 hpf (A) and 72 hpf (B). Bar plots show global changes in relative expression of matn1, klf2a, and mxra5b between 48 hpf uninjected WT controls (blue) and aBTX-injected paralyzed (green) embryos (C), aBTX-injected paralyzed (green) and aBTX-injected ‘Twitching’ (partially recovered, magenta) embryos (D), and between aBTX-injected paralyzed (green) and aBTX-injected, ‘Recovered’ (cyan) embryos (E) at 48 hpf (right). ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001.